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Exam 24: Amino Acids, Peptides, and Proteins

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Question 61

Multiple Choice

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What is the one letter symbol of the amino acid below?

A) G
B) Q
C) W
D) R
E) M

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Question 62

Multiple Choice

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Which of the following indicators is commonly used to visualize bands of amino acids?

A) ninhydrin
B) bromocresol green
C) potassium permanganate
D) vanillin dip

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Question 63

Short Answer

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Complete hydrolysis of a hexapeptide gives 2 Gly, Leu, Phe, Pro, and Tyr. Reaction of the peptide with phenylisothiocyanate gave the phenylthiohydantoin of Pro. Partial hydrolysis of the peptide gave the following fragments: Phe-Gly-Tyr, Gly-Phe-Gly, Pro-Leu-Gly, Leu-Gly-Phe. What is the structure of the peptide?

Show how a monosaccharide such as glucose can attach to a protein structure through the formation of an O-linked glycosidic bond with Ser. (Hint: This structure would be properly called an acetal.)

Which amino acid has an indole ring in its side chain?

Draw the Fischer projection of L-glutamic acid at a pH of 12.

What peptide remains after two sequential Edman degradations of the pentapeptide

Provide the structure of the major organic product which results when L-histidine is treated with benzyl chloroformate.

Which of the following amino acids are classified as essential amino acids? (More than one answer is possible.)

Which of the following amino acids does not contain an aromatic R-group?

Which of the standard amino acids is achiral?

Draw the Fischer projection of L-valine at a pH of 2.

Provide a reasonable synthesis of Gly-Val from the individual amino acids.

The reagent used in the determination of the N-terminal amino acid residue of a peptide is:

Provide a detailed, stepwise mechanism for the Strecker synthesis of racemic alanine from acetaldehyde.

How many standard amino acids are there?

How is the enzyme chymotrypsin used in peptide structure determination?

Chymotrypsin is an enzyme which cleaves a peptide chain at the carboxyl groups of three aromatic amino acid residues. Name these three.

Show the mechanism for the hydrolysis of the following dipeptide?

A polypeptide was treated with a certain enzyme to yield the following amino acid sequences. What enzyme was used to induce the observed fragmentations? Val-Pro-Phe Leu-Ser-Lys-Glu-Trp Arg-Ile-Ser-Ser-Leu-Tyr