Question 1

Which protein forms a complex with a guide RNA to target DNA for cleavage and destruction?

Accepted Answer

A)   RecA
B)   Cas9
C)   EcoRI
D)   Gal4p
E)   GSTF) C) and D)
G) A) and D)

Question 2

The size of the DNA region specifically recognized by Type II restriction enzymes is typically:

Accepted Answer

A)   4 to 6 base pairs.
B)   10 to 15 base pairs.
C)   50 to 60 base pairs.
D)   200 to 300 base pairs.
E)   about the size of an average gene.F) B) and E)
G) A) and B)

Question 3

During their multiple migrations from Africa to the rest of the world, gene flow likely occurred between which populations of early humanoids?

Accepted Answer

A)   between Neanderthals and modern humans
B)   between Denisovans and modern humans
C)   between Neanderthals and Denisovans
D)   All of the answers are correct.
E)   None of the answers is correct.F) A) and E)
G) All of the above

Question 4

Certain restriction enzymes produce cohesive (sticky)  ends. This means that they:

Accepted Answer

A)   cut both DNA strands at the same base pair.
B)   cut in regions of high GC content, leaving ends that can form more hydrogen bonds than ends of high AT content.
C)   make a staggered double-strand cut, leaving ends with a few nucleotides of single-stranded DNA protruding.
D)   make ends that can anneal to cohesive ends generated by any other restriction enzyme.
E)   stick tightly to the ends of the DNA they have cut.F) B) and C)
G) A) and D)

Question 5

For what reason is the mitochondrial genome and the Y chromosome the easiest portions of human DNA to use to trace human evolutionary lineages?

Accepted Answer

A)   All human cells have mitochondria and Y chromosomes.
B)   Mitochondrial DNA and the Y chromosome do not undergo significant meiotic recombination.
C)   The size of the mitochondrial genome and the Y chromosome is small.
D)   Mitochondrial DNA and the Y chromosome exhibit very unstable haplotypes.
E)   Mitochondrial DNA is inherited along both the male and female human evolutionary. lineagesF) B) and E)
G) A) and E)

Question 6

Which technique is NOT used to make a cDNA library?

Accepted Answer

A)   extraction of DNA from the organism you are studying
B)   extraction of mRNA from the organism you are studying
C)   use of reverse transcriptase to copy RNA into DNA
D)   ligation of DNA into a cloning vector
E)   insertion of a cloning vector into cellsF) A) and B)
G) B) and C)

Question 7

What sequences are required in an expression vector (for use with E. coli) that are NOT essential in a cloning plasmid?

Accepted Answer

An expression vector is a type of plasmi...

Question 8

Which statement is NOT correct?

Accepted Answer

A)   Humans and chimpanzees share a common ancestor.
B)   Orangutans are an outgroup when compared with humans and chimpanzees.
C)   Humans and chimpanzees differ over approximately 4% of their genomes.
D)   Humans evolved from chimpanzees.
E)   Humans have 23 pairs of chromosomes, while chimpanzees have 24 pairs of chromosomes.F) A) and E)
G) A) and D)

Question 9

Explain briefly the properties of the plasmid pBR322 that make it so convenient as a vector for cloning fragments of foreign DNA.

Accepted Answer

The plasmid pBR322 has several propertie...

Question 10

Explain the importance of outgroups for understanding genomic lineages.

Accepted Answer

Outgroups are important for understandin...

Question 11

A plasmid that encodes resistance to ampicillin and tetracycline is digested with the restriction enzyme PstI, which cuts the plasmid at a single site in the ampicillin-resistance gene. The DNA is then annealed with a PstI digest of human DNA, ligated, and used to transform E. coli cells. (a) What antibiotic would you put in an agar plate to ensure that the cells of a bacterial colony contain the plasmid? (b) What antibiotic-resistance phenotypes will be found on the plate? (c) Which phenotype will indicate the presence of plasmids that contain human DNA fragments?

Accepted Answer

(a) To ensure that the cells of a bacter...

Question 12

Which statement about Type II restriction enzymes is FALSE?

Accepted Answer

A)   Many make staggered (off-center)  cuts within their recognition sequences.
B)   Some cut DNA to generate blunt ends.
C)   They are part of a bacterial defense system in which foreign DNA is cleaved.
D)   They cleave and ligate DNA.
E)   They cleave DNA only at recognition sequences specific to a given restriction enzyme.F) None of the above
G) A) and C)

Question 13

Describe the difference between selectable markers and screenable markers and their use in cloning experiments.

Accepted Answer

Selectable markers and screenable marker...

Question 14

In the laboratory, recombinant plasmids are commonly introduced into bacterial cells by:

Accepted Answer

A)   electrophoresis-a gentle low-voltage gradient draws the DNA into the cell.
B)   infection with a bacteriophage that carries the plasmid.
C)   microinjection.
D)   mixing plasmids with an extract of broken cells.
E)   transformation-heat shock of the cells incubated with plasmid DNA in the presence of CaCl2.F) A) and E)
G) C) and D)

Question 15

If you wanted to compare the sequence of a gene you isolated in the laboratory with a database of other genetic sequences, which tool would you use?

Accepted Answer

A)   linkage analysis
B)   BLAST
C)   forensic DNA analysis
D)   haplotype identification
E)   quantitative PCRF) A) and E)
G) D) and E)

Question 16

Which way is NOT a means of inserting DNA into a cell?

Accepted Answer

A)   transformation
B)   transfection
C)   transduction
D)   electroporation
E)   ligationF) C) and E)
G) B) and E)

Question 17

A good expression vector for protein expression in E. coli should have which feature(s) ?

Accepted Answer

A)   an origin of replication
B)   a selectable marker
C)   a promoter, operator, and ribosome-binding site
D)   All of the answers are correct.
E)   None of the answers is correct.F) A) and C)
G) B) and D)

Question 18

Which would you expect to be larger, the percentage of the human genome that is translated into protein or the percentage of the genome of a bacterium that is translated into protein? Why?

Accepted Answer

The percentage of the human genome that ...

Question 19

What is/are the distinguishing feature(s) of a shuttle vector?

Accepted Answer

A shuttle vector is a type of plasmid or...

Question 20

A convenient cloning vector with which to introduce foreign DNA into E. coli is a(n) :

Accepted Answer

A)   E. coli chromosome.
B)   messenger RNA.
C)   plasmid.
D)   yeast "ARS" sequence.
E)   yeast transposable element.F) B) and D)
G) A) and C)

Exam 9: DNA-Based Information Technologies

The size of the DNA region specifically recognized by Type II restriction enzymes is typically:

Correct Answer
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Explain the importance of outgroups for understanding genomic lineages.

Correct Answer
Answered by ExamLex AI
Outgroups are important for understandin...
View Answer

During their multiple migrations from Africa to the rest of the world, gene flow likely occurred between which populations of early humanoids?

Correct Answer
verified

Describe the difference between selectable markers and screenable markers and their use in cloning experiments.

Correct Answer
Answered by ExamLex AI
Selectable markers and screenable marker...
View Answer

Which statement is NOT correct?

Correct Answer
verified

A convenient cloning vector with which to introduce foreign DNA into E. coli is a(n) :

Correct Answer
verified

Which way is NOT a means of inserting DNA into a cell?

Correct Answer
verified

Explain briefly the properties of the plasmid pBR322 that make it so convenient as a vector for cloning fragments of foreign DNA.

Correct Answer
Answered by ExamLex AI
The plasmid pBR322 has several propertie...
View Answer

Which would you expect to be larger, the percentage of the human genome that is translated into protein or the percentage of the genome of a bacterium that is translated into protein? Why?

Correct Answer
Answered by ExamLex AI
The percentage of the human genome that ...
View Answer

Certain restriction enzymes produce cohesive (sticky) ends. This means that they:

Correct Answer
verified

Which technique is NOT used to make a cDNA library?

Correct Answer
verified

Correct Answer
Answered by ExamLex AI
(a) To ensure that the cells of a bacter...
View Answer

Which statement about Type II restriction enzymes is FALSE?

Correct Answer
verified

If you wanted to compare the sequence of a gene you isolated in the laboratory with a database of other genetic sequences, which tool would you use?

Correct Answer
verified

What sequences are required in an expression vector (for use with E. coli) that are NOT essential in a cloning plasmid?

Correct Answer
Answered by ExamLex AI
An expression vector is a type of plasmi...
View Answer

For what reason is the mitochondrial genome and the Y chromosome the easiest portions of human DNA to use to trace human evolutionary lineages?

Correct Answer
verified

Which protein forms a complex with a guide RNA to target DNA for cleavage and destruction?

Correct Answer
verified

A good expression vector for protein expression in E. coli should have which feature(s) ?

Correct Answer
verified

What is/are the distinguishing feature(s) of a shuttle vector?

Correct Answer
Answered by ExamLex AI
A shuttle vector is a type of plasmid or...
View Answer

In the laboratory, recombinant plasmids are commonly introduced into bacterial cells by:

Correct Answer
verified

Exam 9: DNA-Based Information Technologies

The size of the DNA region specifically recognized by Type II restriction enzymes is typically:

Correct AnswerverifiedShow Answer

Explain the importance of outgroups for understanding genomic lineages.

Correct AnswerAnswered by ExamLex AIOutgroups are important for understandin...View AnswerShow Answer

During their multiple migrations from Africa to the rest of the world, gene flow likely occurred between which populations of early humanoids?

Correct AnswerverifiedShow Answer

Describe the difference between selectable markers and screenable markers and their use in cloning experiments.

Correct AnswerAnswered by ExamLex AISelectable markers and screenable marker...View AnswerShow Answer

Which statement is NOT correct?

Correct AnswerverifiedShow Answer

A convenient cloning vector with which to introduce foreign DNA into E. coli is a(n) :

Correct AnswerverifiedShow Answer

Which way is NOT a means of inserting DNA into a cell?

Correct AnswerverifiedShow Answer

Explain briefly the properties of the plasmid pBR322 that make it so convenient as a vector for cloning fragments of foreign DNA.

Correct AnswerAnswered by ExamLex AIThe plasmid pBR322 has several propertie...View AnswerShow Answer

Which would you expect to be larger, the percentage of the human genome that is translated into protein or the percentage of the genome of a bacterium that is translated into protein? Why?

Correct AnswerAnswered by ExamLex AIThe percentage of the human genome that ...View AnswerShow Answer

Certain restriction enzymes produce cohesive (sticky) ends. This means that they:

Correct AnswerverifiedShow Answer

Which technique is NOT used to make a cDNA library?

Correct AnswerverifiedShow Answer

Correct AnswerAnswered by ExamLex AI(a) To ensure that the cells of a bacter...View AnswerShow Answer

Which statement about Type II restriction enzymes is FALSE?

Correct AnswerverifiedShow Answer

If you wanted to compare the sequence of a gene you isolated in the laboratory with a database of other genetic sequences, which tool would you use?

Correct AnswerverifiedShow Answer

What sequences are required in an expression vector (for use with E. coli) that are NOT essential in a cloning plasmid?

Correct AnswerAnswered by ExamLex AIAn expression vector is a type of plasmi...View AnswerShow Answer

For what reason is the mitochondrial genome and the Y chromosome the easiest portions of human DNA to use to trace human evolutionary lineages?

Correct AnswerverifiedShow Answer

Which protein forms a complex with a guide RNA to target DNA for cleavage and destruction?

Correct AnswerverifiedShow Answer

A good expression vector for protein expression in E. coli should have which feature(s) ?

Correct AnswerverifiedShow Answer

What is/are the distinguishing feature(s) of a shuttle vector?

Correct AnswerAnswered by ExamLex AIA shuttle vector is a type of plasmid or...View AnswerShow Answer

In the laboratory, recombinant plasmids are commonly introduced into bacterial cells by:

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
Answered by ExamLex AI
Outgroups are important for understandin...
View Answer

Correct Answer
verified

Correct Answer
Answered by ExamLex AI
Selectable markers and screenable marker...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
Answered by ExamLex AI
The plasmid pBR322 has several propertie...
View Answer

Correct Answer
Answered by ExamLex AI
The percentage of the human genome that ...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
Answered by ExamLex AI
(a) To ensure that the cells of a bacter...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
Answered by ExamLex AI
An expression vector is a type of plasmi...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
Answered by ExamLex AI
A shuttle vector is a type of plasmid or...
View Answer

Correct Answer
verified