Question 1

A medium that is gel-like has less agar in it compared to a solid medium.

Accepted Answer

A) True 
 B)False

Question 2

A pure culture contains only

Accepted Answer

A)  one species of microorganism.
B)  bacteria.
C)  a variety of microbes from one source.
D)  All of the choices are correct.
E)  None of the choices are correct.F) A) and B)
G) A) and C)

Question 3

Brain-heart infusion, trypticase soy agar (TSA) , and nutrient agar are all examples of which type of media?

Accepted Answer

A)  synthetic
B)  reducing
C)  enriched
D)  nonsynthetic
E)  selectiveF) A) and B)
G) A) and E)

Question 4

The wavelength of light used plus the numerical aperture governs

Accepted Answer

A)  illumination.
B)  resolution.
C)  magnification.
D)  size of the field.
E)  All of the choices are correct.F) A) and D)
G) D) and E)

Question 5

A bacterial species that grows on blood agar but will not grow on trypticase soy agar is termed an anaerobe.

Accepted Answer

A) True 
 B)False

Question 6

Scanning tunneling and atomic force microscopes are used to image the detailed structure of biological molecules.

Accepted Answer

A) True 
 B)False

Question 7

A microbiologist makes a fixed smear of bacterial cells and stains them with Loeffler's methylene blue.All the cells appear blue under the oil lens.This is an example of

Accepted Answer

A)  negative staining.
B)  using an acidic dye.
C)  simple staining.
D)  using the acid-fast stain.
E)  capsule staining.F) A) and D)
G) C) and D)

Question 8

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Accepted Answer

A)  differential
B)  selective
C)  enumeration
D)  enriched
E)  reducingF) C) and E)
G) C) and D)

Question 9

The _____ of the microscope holds and allows selection of the objective lenses.

Accepted Answer

The answer of The _____ of the microscope holds and...

Question 10

The specimen preparation that is best for viewing cell motility is

Accepted Answer

A)  hanging drop.
B)  fixed stained smear.
C)  Gram stain.
D)  negative stain.
E)  flagellar stain.F) B) and E)
G) A) and C)

Question 11

Explain how and why immersion oil increases resolution but not magnification when using the 100X objective.

Accepted Answer

Immersion oil increases resolution but n...

Question 12

Which method often results in colonies developing down throughout the agar and some colonies on the surface?

Accepted Answer

A)  streak plate
B)  spread plate
C)  pour plate
D)  All of the choices are correct
E)  None of the choices are correctF) B) and E)
G) A) and B)

Question 13

A microbiologist inoculates Staphylococcus aureus into a culture medium.Following incubation, both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture.What is the most likely explanation?

Accepted Answer

A)  The microbiologist used too much inoculum.
B)  The culture is contaminated.
C)  The incubation temperature was incorrect.
D)  The culture medium must be selective.
E)  The culture medium must be differential.F) A) and E)
G) A) and D)

Question 14

Which microscope bombards a whole, metal-coated specimen with electrons moving back and forth over it?

Accepted Answer

A)  fluorescence
B)  differential interference contrast
C)  scanning electron
D)  transmission electron
E)  phase-contrastF) None of the above
G) C) and D)

Question 15

The three physical forms of laboratory media are: solid, semisolid, and _____.

Accepted Answer

The answer of The three physical forms of laboratory media...

Question 16

Which microscope shows cells against a bright background and the intracellular structures of unstained cells based on their varying densities?

Accepted Answer

A)  bright-field
B)  dark-field
C)  phase-contrast
D)  fluorescence
E)  electronF) A) and E)
G) D) and E)

Question 17

All of the following are examples of different types of microbiological media except

Accepted Answer

A)  broth.
B)  enriched.
C)  agar.
D)  petri dish.
E)  gelatin.F) C) and E)
G) B) and D)

Question 18

The bending of light rays as they pass from one medium to another is called refraction.

Accepted Answer

A) True 
 B)False

Question 19

_____ dyes have a negative charge on the chromophore and are repelled by bacterial cells.

Accepted Answer

The answer of _____ dyes have a negative charge on...

Question 20

Which of the following reagents reacts with crystal violet as the mordant?

Accepted Answer

A)  Crystal violet
B)  Gram's iodine
C)  95% ethyl alcohol
D)  SafraninE) All of the above
F) A) and B)

Exam 3: Tools of the Laboratory: Methods of Studying Microorganisms

The bending of light rays as they pass from one medium to another is called refraction.

Correct AnswerverifiedShow Answer

Which of the following reagents reacts with crystal violet as the mordant?

Correct AnswerverifiedShow Answer

Which method often results in colonies developing down throughout the agar and some colonies on the surface?

Correct AnswerverifiedShow Answer

Which microscope bombards a whole, metal-coated specimen with electrons moving back and forth over it?

Correct AnswerverifiedShow Answer

Scanning tunneling and atomic force microscopes are used to image the detailed structure of biological molecules.

Correct AnswerverifiedShow Answer

The wavelength of light used plus the numerical aperture governs

Correct AnswerverifiedShow Answer

All of the following are examples of different types of microbiological media except

Correct AnswerverifiedShow Answer

Which microscope shows cells against a bright background and the intracellular structures of unstained cells based on their varying densities?

Correct AnswerverifiedShow Answer

A medium that is gel-like has less agar in it compared to a solid medium.

Correct AnswerverifiedShow Answer

_____ dyes have a negative charge on the chromophore and are repelled by bacterial cells.

Correct AnswerverifiedShow Answer

The three physical forms of laboratory media are: solid, semisolid, and _____.

Correct AnswerverifiedShow Answer

A microbiologist makes a fixed smear of bacterial cells and stains them with Loeffler's methylene blue.All the cells appear blue under the oil lens.This is an example of

Correct AnswerverifiedShow Answer

A bacterial species that grows on blood agar but will not grow on trypticase soy agar is termed an anaerobe.

Correct AnswerverifiedShow Answer

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Correct AnswerverifiedShow Answer

Brain-heart infusion, trypticase soy agar (TSA) , and nutrient agar are all examples of which type of media?

Correct AnswerverifiedShow Answer

The specimen preparation that is best for viewing cell motility is

Correct AnswerverifiedShow Answer

A microbiologist inoculates Staphylococcus aureus into a culture medium.Following incubation, both Staphylococcus aureus and Staphylococcus epidermidis are determined to be growing in this culture.What is the most likely explanation?

Correct AnswerverifiedShow Answer

Explain how and why immersion oil increases resolution but not magnification when using the 100X objective.

Correct AnswerAnswered by ExamLex AIImmersion oil increases resolution but n...View AnswerShow Answer

The _____ of the microscope holds and allows selection of the objective lenses.

Correct AnswerverifiedShow Answer

A pure culture contains only

Correct AnswerverifiedShow Answer

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Immersion oil increases resolution but n...
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