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Recombinant DNA-based products are slow to reach the marketplace because of


A) the adverse effects of gene cloning.
B) the lack of technological advances in gene cloning.
C) the lack of safety involved in such methods.
D) the cost and duration of research.

E) None of the above
F) B) and D)

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Restriction enzymes are useful in creating recombinant DNA molecules because they


A) generate "sticky ends" by cutting at the same sequence in any DNA source.
B) are found only in human cells,where they naturally mend broken DNA strands.
C) are used to encapsulate and transport foreign DNA into cells of the target organism.
D) stimulate DNA amplification so that the desired cells or their products can be scaled up.

E) C) and D)
F) A) and D)

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Most of the effort involved in recombinant DNA technology involves


A) identifying and separating cells that contain the gene of interest.
B) finding a restriction enzyme to cut DNA from a donor cell.
C) identifying a cloning vector that can hold a gene.
D) finding uses for recombined DNA.

E) C) and D)
F) None of the above

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_____,a recombinant clotbusting drug,is used to limit damage to heart muscle by restoring blood flow.


A) Insulin
B) Tissue plasminogen activator
C) Interleukin-2
D) Glucocerebrosidase

E) B) and C)
F) All of the above

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A naturally occurring,small,circle of DNA used as a vector to transmit DNA is a


A) plasmid.
B) prion.
C) lipofectin.
D) ring chromosome.

E) A) and B)
F) A) and C)

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Transgenic organisms carry the transgene in


A) every cell.
B) gametes only.
C) somatic cells only.
D) the cell in which it was originally introduceD.

E) A) and B)
F) A) and C)

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Both gene silencing and genome editing techniques are limited by "off-target effects"-doing something other than what we want them to do.

A) True
B) False

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_____ is a gene silencing technique that is based on the fact that RNA molecules can fold into short,double-stranded regions where the base sequence is complementary.


A) RNA repair
B) RNA amplification
C) RNA replication
D) RNA interference

E) B) and C)
F) B) and D)

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One of the science-related concerns associated with the use of genetically modified (GM) foods is that


A) overreliance on GM crops may lead to genetic uniformity.
B) GM foods are not natural.
C) genetic modification degrades the nutritional value of food crops.
D) buffer zones of GM crops halt the pollination of non-GM crops.

E) C) and D)
F) None of the above

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Transgenic farm animals have not gained importance as sources of pharmaceuticals because


A) they are too difficult to maintain.
B) they are more susceptible to cancer and other diseases.
C) they produce diluted forms of pharmaceuticals.
D) they carry multiple viral strains.

E) A) and B)
F) A) and C)

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A drug produced using recombinant DNA technology that is used illegally by athletes is


A) insulin.
B) streptokinase.
C) tissue plasminogen activator.
D) erythropoietin.

E) A) and B)
F) A) and C)

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One of the earliest uses of biotechnology was


A) creating transgenic organisms.
B) creating bacteria that synthesized human insulin.
C) extracting and using biochemicals from microorganisms.
D) using yeast to ferment fruit into wine.

E) B) and D)
F) B) and C)

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Patent law as it pertains to biotechnology has had to change in recent years in response to


A) the changeable nature of the genetic code.
B) the number of people asking for genetic tests.
C) the increasing number of human genes.
D) the greatly accelerated speed of DNA sequencing.

E) None of the above
F) All of the above

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Using transgenic animal models has its limitations because


A) prokaryotic cells are generally better at producing human proteins.
B) bacterial pathogens that affect humans do not affect animals.
C) the level of gene expression is significantly higher in animals.
D) researchers cannot control where a transgene inserts itself into a genome.

E) All of the above
F) A) and D)

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Courts allow patenting complementary DNA (cDNA) because


A) its exact sequence is not in the genome of an organism.
B) it cannot be synthesized in a laboratory.
C) it is present only in adult humans.
D) it represents only the introns of a gene.

E) A) and B)
F) B) and D)

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The first patent on a living organism was granted in


A) 1790,when the U.S.passed patent law.
B) 1873,for Louis Pasteur's use of yeast.
C) 1900,when Mendel's laws were rediscovered.
D) 1978,when modern biotechnology began with recombinant insulin.

E) A) and D)
F) All of the above

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Structures used in recombinant DNA technology to carry the DNA from the cells of one species to the cells of another species are


A) cloning vectors.
B) restriction enzymes.
C) sticky ends.
D) donor DNA.

E) None of the above
F) All of the above

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A gene expression microarray has


A) an entire genome of DNA,cut into hundreds of pieces,attached to a small plastic or glass square.
B) short pieces of DNA of known sequence attached to a small plastic or glass square.
C) short pieces of RNA of known sequence attached to a small plastic or glass square.
D) amino acids attached to a small plastic or glass square.

E) A) and C)
F) A) and B)

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In 2010,a federal judge in the U.S.ruled seven patents on the BRCA1 and BRCA2 genes had been improperly granted.

A) True
B) False

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Proteins isolated from bacteria and used in recombinant DNA technology to cut DNA at specific sequences are


A) restriction enzymes.
B) bacteriophage enzymes.
C) plasmids.
D) methylating enzymes.

E) A) and C)
F) All of the above

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