Question 1

Syphilis can be diagnosed by the

Accepted Answer

A)  Weil-Felix reaction.
B)  rapid plasma reagin test.
C)  Widal test.
D)  latex agglutination test.
E)  antistreptolysin O test.F) C) and E)
G) All of the above

Question 2

A direct immunofluorescence test involves a/an

Accepted Answer

A)  precipitation reaction.
B)  agglutination reaction.
C)  known antibody binding antigen
D)  lysis reaction.E) All of the above
F) C) and D)

Question 3

Antibody testing requires

Accepted Answer

A)  only a known antigen.
B)  only a known antibody.
C)  both a known antigen and a known antibody.
D)  either a known antigen or a known antibody.E) A) and B)
F) None of the above

Question 4

You would like to identify as many bacterial species as possible in the human mouth.Why is biochemical testing not the most efficient and accurate method of completing this task?

Accepted Answer

A)  Since you do not know the growth characteristics and metabolic properties of the bacteria in the mouth,you cannot determine all varieties of media to use for isolation and culture.
B)  Bacteria in the mouth are difficult to grow because of the adverse conditions in the oral habitats.
C)  Bacteriological media is very expensive,particularly when you would have to use a variety of types to identify the different bacteria.
D)  Biochemical testing,using a variety of media,is inaccurate.It has been superceded by more advanced testing procedures.E) B) and C)
F) A) and B)

Question 5

Staphylococcus aureus can be identified by mixing the organism with specific antibody,and visually recognizing a clumping reaction between the antigen and the antibody.This is a(n) _______ test.

Accepted Answer

A)  microarray
B)  biochemical
C)  agglutination
D)  ELISAE) A) and D)
F) All of the above

Question 6

Phage typing is useful in identifying

Accepted Answer

A)  Treponema pallidum.
B)  Mycobacterium leprae.
C)  Streptococcus pyogenes.
D)  Salmonella.
E)  ClostridiumF) D) and E)
G) B) and D)

Question 7

The VRDL test detects

Accepted Answer

A)  syphilis.
B)  Rickettsia.
C)  Salmonella.
D)  Candida.E) A) and B)
F) None of the above

Question 8

Your daughter probably has strep throat,caused by Streptococcus pyogenes.The traditional method of diagnosis involves culturing on blood agar,but this process is time consuming.Your doctor wants a more rapid identification of the infectious agent using a specimen directly obtained from the patient's throat using a test that can be read in his office,without having to culture and isolate the organism.He would likely choose the:

Accepted Answer

A)  direct fluorescence test.
B)  Western Blot test.
C)  immunochromatographic test.
D)  pulse-field gel electrophoresis.E) B) and C)
F) A) and D)

Question 9

A positive direct ELISA result requires

Accepted Answer

A)  one known antibody,one unknown antibody,and an unknown antigen.
B)  one known antibody,one unknown antibody,and a known antigen.
C)  two known antibodies and one known antigen.
D)  two known antibodies and one unknown antigen.E) A) and D)
F) C) and D)

Question 10

Antibiotics are often added to biochemical media to restrict the growth to only those organisms that are being looked for in the specimens.

Accepted Answer

A) True 
 B)False

Question 11

Serological testing relies upon

Accepted Answer

A)  the specificity of the Fc region of antibodies.
B)  the specificity of the variable regions of antibodies.
C)  a patient who is not immunocompromised.
D)  a very high viral or bacterial load in the patient.E) A) and B)
F) A) and C)

Question 12

Which test cannot be used for identification of a person's HIV status?

Accepted Answer

A)  ELISA
B)  Western Blot
C)  immunochromatographic testing
D)  biochemical testsE) None of the above
F) B) and C)

Question 13

Serological tests should have low sensitivity and specificity.

Accepted Answer

A) True 
 B)False

Question 14

Serological testing always involves reactions between specific antibody and antigen.

Accepted Answer

A) True 
 B)False

Question 15

The Western blot test is confirmatory for HIV because it

Accepted Answer

A)  is more sensitive than the ELISA.
B)  has fewer false positives than the ELISA.
C)  requires electrophoresis,whereas the ELISA does not.
D)  uses an indicator of some sort to visualize the antibody.
E)  All of the choices are correct.F) B) and E)
G) B) and D)

Question 16

Serotyping of bacteria cannot be used to identify this component:

Accepted Answer

A)  cell wall.
B)  flagella.
C)  capsule.
D)  DNA.E) A) and D)
F) A) and C)

Question 17

The polymerase chain reaction requires endonuclease to cut samples of DNA.

Accepted Answer

A) True 
 B)False

Question 18

The radioimmunoassay test is used frequently for

Accepted Answer

A)  identification of bacteria.
B)  determining levels of hormones.
C)  separation of DNA fragments.
D)  identification of viruses.E) A) and B)
F) None of the above

Question 19

The primary advantage of genotypic analysis for identification is

Accepted Answer

A)  it is easier than any other method.
B)  it is less expensive than other methods.
C)  it is widely available.
D)  culturing of the organism is not required.E) None of the above
F) All of the above

Question 20

The urine specimen obtained from a patient went to the microbiology lab for a bacterial culture and antibiotic sensitivity testing.The specimen sat out on the processing counter for a couple of hours before being cultured.Twenty fours hours later,the lab report goes into the computer---over 100,000 bacterial cells per milliliter of urine.Which statement is an accurate assessment of these results?

Accepted Answer

A)  The bacterial count is not accurate since the culture sat without refrigeration.
B)  Not all bacteria will grow on bacteriological media,so the total count is grossly underestimated.
C)  Urine is a sterile fluid as it leaves the body,so it is likely that the specimen was mishandled by the nursing staff when the sample was obtained.
D)  Urine samples have to be taken from the kidney in order to get a true bacterial count.E) B) and C)
F) A) and D)

Exam 17: Diagnosing Infections

Serological testing relies upon

Correct Answer
verified

The radioimmunoassay test is used frequently for

Correct Answer
verified

Correct Answer
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Staphylococcus aureus can be identified by mixing the organism with specific antibody,and visually recognizing a clumping reaction between the antigen and the antibody.This is a(n) _______ test.

Correct Answer
verified

Correct Answer
verified

Antibody testing requires

Correct Answer
verified

Syphilis can be diagnosed by the

Correct Answer
verified

Which test cannot be used for identification of a person's HIV status?

Correct Answer
verified

A direct immunofluorescence test involves a/an

Correct Answer
verified

Serological testing always involves reactions between specific antibody and antigen.

Correct Answer
verified

The polymerase chain reaction requires endonuclease to cut samples of DNA.

Correct Answer
verified

Serotyping of bacteria cannot be used to identify this component:

Correct Answer
verified

You would like to identify as many bacterial species as possible in the human mouth.Why is biochemical testing not the most efficient and accurate method of completing this task?

Correct Answer
verified

The VRDL test detects

Correct Answer
verified

Antibiotics are often added to biochemical media to restrict the growth to only those organisms that are being looked for in the specimens.

Correct Answer
verified

The primary advantage of genotypic analysis for identification is

Correct Answer
verified

Phage typing is useful in identifying

Correct Answer
verified

The Western blot test is confirmatory for HIV because it

Correct Answer
verified

A positive direct ELISA result requires

Correct Answer
verified

Serological tests should have low sensitivity and specificity.

Correct Answer
verified

Exam 17: Diagnosing Infections

Serological testing relies upon

Correct AnswerverifiedShow Answer

The radioimmunoassay test is used frequently for

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Staphylococcus aureus can be identified by mixing the organism with specific antibody,and visually recognizing a clumping reaction between the antigen and the antibody.This is a(n) _______ test.

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Antibody testing requires

Correct AnswerverifiedShow Answer

Syphilis can be diagnosed by the

Correct AnswerverifiedShow Answer

Which test cannot be used for identification of a person's HIV status?

Correct AnswerverifiedShow Answer

A direct immunofluorescence test involves a/an

Correct AnswerverifiedShow Answer

Serological testing always involves reactions between specific antibody and antigen.

Correct AnswerverifiedShow Answer

The polymerase chain reaction requires endonuclease to cut samples of DNA.

Correct AnswerverifiedShow Answer

Serotyping of bacteria cannot be used to identify this component:

Correct AnswerverifiedShow Answer

You would like to identify as many bacterial species as possible in the human mouth.Why is biochemical testing not the most efficient and accurate method of completing this task?

Correct AnswerverifiedShow Answer

The VRDL test detects

Correct AnswerverifiedShow Answer

Antibiotics are often added to biochemical media to restrict the growth to only those organisms that are being looked for in the specimens.

Correct AnswerverifiedShow Answer

The primary advantage of genotypic analysis for identification is

Correct AnswerverifiedShow Answer

Phage typing is useful in identifying

Correct AnswerverifiedShow Answer

The Western blot test is confirmatory for HIV because it

Correct AnswerverifiedShow Answer

A positive direct ELISA result requires

Correct AnswerverifiedShow Answer

Serological tests should have low sensitivity and specificity.

Correct AnswerverifiedShow Answer

Correct Answer
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