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The following steps are required for making cheese.What is the second step,which helps to provide the characteristic flavors and aromas of the cheese?


A) enzymatic coagulation of milk
B) fermentation of curd
C) inoculation with lactic acid bacteria
D) inoculation with Penicillium
E) separation of curds and whey

F) A) and C)
G) B) and D)

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Aseptic packaging utilizes which of the following to sterilize laminated paper or plastic prior to filling with food?


A) hot HCl solution
B) gamma radiation
C) hot hydrogen peroxide solution
D) X rays
E) autoclave

F) B) and E)
G) A) and E)

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High-pressure processing of foods such as deli meats and precooked chicken strips targets pathogens such as Salmonella and Listeria.

A) True
B) False

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Explain how ionizing radiation can be used to preserve foods.Discuss its advantage over canning as a method of preservation.

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Figure 28.2 Figure 28.2    -Which of the growth curves in Figure 28.2 is indicating the culture has reached the trophophase? A) a B) b C) c D) d E) e -Which of the growth curves in Figure 28.2 is indicating the culture has reached the trophophase?


A) a
B) b
C) c
D) d
E) e

F) A) and E)
G) A) and D)

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All of the following are advantages of a bioreactor instead of a flask culture EXCEPT


A) larger culture volumes can be grown.
B) instrumentation for monitoring environmental conditions.
C) uniform aeration and mixing.
D) aseptic sampling.
E) None of the answers is correct;all of these are advantages of using a bioreactor instead of a flask culture.

F) A) and C)
G) A) and B)

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Commercial sterilization of processed canned foods uses a 12D treatment which is based upon a theoretical population of 10¹² C.botulinum endospores in a can of food.After treatment,there would theoretically be how many endospores left?


A) 120 endospores
B) 10 endospores
C) 100 endospores
D) 12 endospores
E) 1 endospore

F) C) and D)
G) C) and E)

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Figure 28.2 Figure 28.2    -Which of the growth curves in Figure 28.2 is indicating the culture has reached the idiophase? A) a B) b C) c D) d E) e -Which of the growth curves in Figure 28.2 is indicating the culture has reached the idiophase?


A) a
B) b
C) c
D) d
E) e

F) B) and D)
G) All of the above

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Microbial products can be improved by all of the following EXCEPT


A) isolating new strains.
B) mutating existing strains.
C) genetically modifying strains.
D) modifying culture conditions.
E) sterilization.

F) A) and B)
G) D) and E)

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The longer interior-ripened cheeses are permitted to ripen,the milder the taste of the cheese.

A) True
B) False

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Use of fossil fuels is beginning to change in modern society.Describe what a biofuel is,and identify/discuss the pros and cons of biofuels.

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If an industrial microbiologist works with Acidithiobacillus ferrooxidans,he is likely to be involved in copper mining.

A) True
B) False

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Figure 28.1 Figure 28.1    -In Figure 28.1,assume the cells are your desired product and you want to maintain a continuous culture.Remember,the top lines/markings indicate the different phases observed in the curve below them.When would you harvest cells to maximize your yield? A) a B) b C) c D) d -In Figure 28.1,assume the cells are your desired product and you want to maintain a continuous culture.Remember,the top lines/markings indicate the different phases observed in the curve below them.When would you harvest cells to maximize your yield?


A) a
B) b
C) c
D) d

E) B) and C)
F) A) and C)

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Microorganisms themselves are commercial products.Which of the following microbes is available as (or in) a product sold in retail stores?


A) Bacillus thuringiensis
B) Lactobacillus
C) Rhizobium
D) Saccharomyces
E) All of these organisms can be found in various commercial products.

F) A) and D)
G) D) and E)

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Which one of the following reactions makes wine less acidic,and is important for good flavor when grapes with higher acidity are used?


A) sugar → ethanol
B) ethanol → acetic acid
C) malic acid → lactic acid
D) carbon dioxide → sucrose
E) sugar → CO₂ + H₂O

F) C) and E)
G) B) and C)

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The blue-green clumps observed in Roquefort cheese are a result of mold growth.

A) True
B) False

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What will be produced if wine is aerated and inoculated with bacteria of the genera Acetobacter and Gluconobacter?


A) ethanol + CO₂
B) CO₂ + H₂O
C) CH₄
D) acetic acid
E) None of the answers is correct.

F) D) and E)
G) B) and D)

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All of the following are industrial products produced using microbial fermentations EXCEPT


A) aspartic acid.
B) citric acid.
C) glutamic acid.
D) riboflavin.
E) saccharin.

F) D) and E)
G) A) and D)

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All of the following foods are produced using microbial fermentations EXCEPT


A) sauerkraut.
B) ketchup.
C) sour cream.
D) yogurt.
E) coffee.

F) B) and D)
G) A) and C)

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Spoilage of canned foods due to inadequate processing,NOT accompanied by gas production,is


A) thermophilic anaerobic spoilage.
B) flat sour spoilage.
C) spoilage by mesophilic bacteria.
D) caused by acid-tolerant fungi.
E) putrefactive anaerobic spoilage.

F) A) and B)
G) None of the above

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