Question 1

A microbiologist makes a ﬁxed smear of bacterial cells and stains it with methylene blue. All cells appear blue under the oil immersion lens. This is an example of ______.

Accepted Answer

A)   capsule staining
B)   the acid-fast stain
C)   negative staining
D)   simple staining
E)   using an acidic dyeF) A) and B)
G) A) and C)

Question 2

A scanning confocal microscope _______.

Accepted Answer

A)   uses ultraviolet light to form a specimen image
B)   produces specimen images on electron micrographs
C)   uses dyes that emit visible light when excited by ultraviolet radiation
D)   scans narrow planes and can build a three-dimensional image of the specimenE) A) and B)
F) A) and C)

Question 3

Some microbes are not capable of growing on artiﬁcial media as pure cultures.

Accepted Answer

A) True 
 B)False

Question 4

Magni?cation is achieved in a compound microscope through the initial magni?cation of the specimen by the _____ lens. This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.

Accepted Answer

A)   objective; ocular
B)   ocular; objective
C)   ocular; oil immersion
D)   scanning; objectiveE) A) and B)
F) A) and C)

Question 5

A pure culture contains _______.

Accepted Answer

A)   microbes from a single source
B)   only bacteria
C)   a variety of species from the same genus
D)   only one identiﬁed species of microorganismE) A) and D)
F) A) and C)

Question 6

The type of microscopy in which you would observe brightly illuminated specimens against a black background is ______.

Accepted Answer

A)   phase-contrast
B)   dark-ﬁeld
C)   confocal
D)   electron
E)   ﬂuorescenceF) A) and B)
G) B) and E)

Question 7

Which type of microscope bombards a whole, metal-coated specimen with electrons moving back and forth over it?

Accepted Answer

A)   Phase-contrast
B)   Scanning electron
C)   Differential interference contrast
D)   Fluorescence
E)   Transmission electronF) C) and D)
G) A) and B)

Question 8

The correct microbiological term for the sample of specimen that is put into a nutrient medium to produce a culture is ______.

Accepted Answer

A)   colony
B)   loop
C)   inoculum
D)   streakE) None of the above
F) B) and C)

Question 9

Mixed cultures are also referred to as contaminated cultures.

Accepted Answer

A) True 
 B)False

Question 10

The bending of light rays as they pass from one medium to another is called refraction.

Accepted Answer

A) True 
 B)False

Question 11

Basic dyes are _______.

Accepted Answer

A)   used in negative staining
B)   anionic
C)   repelled by cells
D)   dyes such as India ink and nigrosin
E)   attracted to the acidic surface of bacterial cellsF) A) and C)
G) A) and E)

Question 12

A medium that is gel-like has less agar in it compared to a solid medium.

Accepted Answer

A) True 
 B)False

Question 13

The specimen preparation technique that is best for viewing cell motility is the ________.

Accepted Answer

A)   hanging drop
B)   ﬂagellar stain
C)   Gram stain
D)   ﬁxed, stained smear
E)   negative stainF) B) and C)
G) A) and E)

Question 14

What is the term for a culture made from one isolated colony?

Accepted Answer

A)   Axenic
B)   Mixed
C)   Pure
D)   Both axenic and mixed are correct.
E)   Both axenic and pure are correct.F) C) and D)
G) B) and E)

Question 15

A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture medium. Following incubation, only the E. coli grows in the culture. What is the most likely explanation?

Accepted Answer

A)   The microbiologist used too much inoculum.
B)   The culture was contaminated.
C)   The incubation temperature was incorrect.
D)   The culture medium was differential.
E)   The culture medium was selective.F) B) and E)
G) B) and C)

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

A medium that is gel-like has less agar in it compared to a solid medium.

Correct Answer
verified

A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture medium. Following incubation, only the E. coli grows in the culture. What is the most likely explanation?

Correct Answer
verified

Mixed cultures are also referred to as contaminated cultures.

Correct Answer
verified

Some microbes are not capable of growing on artiﬁcial media as pure cultures.

Correct Answer
verified

A scanning confocal microscope _______.

Correct Answer
verified

Magni?cation is achieved in a compound microscope through the initial magni?cation of the specimen by the _ lens. This image is then projected to the _ lens that will further magnify the specimen to form a virtual image received by the eye.

Correct Answer
verified

The type of microscopy in which you would observe brightly illuminated specimens against a black background is ______.

Correct Answer
verified

The correct microbiological term for the sample of specimen that is put into a nutrient medium to produce a culture is ______.

Correct Answer
verified

The bending of light rays as they pass from one medium to another is called refraction.

Correct Answer
verified

A microbiologist makes a ﬁxed smear of bacterial cells and stains it with methylene blue. All cells appear blue under the oil immersion lens. This is an example of ______.

Correct Answer
verified

The specimen preparation technique that is best for viewing cell motility is the ________.

Correct Answer
verified

Basic dyes are _______.

Correct Answer
verified

A pure culture contains _______.

Correct Answer
verified

Which type of microscope bombards a whole, metal-coated specimen with electrons moving back and forth over it?

Correct Answer
verified

What is the term for a culture made from one isolated colony?

Correct Answer
verified

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

A medium that is gel-like has less agar in it compared to a solid medium.

Correct AnswerverifiedShow Answer

A microbiologist inoculates Staphylococcus epidermidis and Escherichia coli into a culture medium. Following incubation, only the E. coli grows in the culture. What is the most likely explanation?

Correct AnswerverifiedShow Answer

Mixed cultures are also referred to as contaminated cultures.

Correct AnswerverifiedShow Answer

Some microbes are not capable of growing on artiﬁcial media as pure cultures.

Correct AnswerverifiedShow Answer

A scanning confocal microscope _______.

Correct AnswerverifiedShow Answer

Magni?cation is achieved in a compound microscope through the initial magni?cation of the specimen by the _____ lens. This image is then projected to the _____ lens that will further magnify the specimen to form a virtual image received by the eye.

Correct AnswerverifiedShow Answer

The type of microscopy in which you would observe brightly illuminated specimens against a black background is ______.

Correct AnswerverifiedShow Answer

The correct microbiological term for the sample of specimen that is put into a nutrient medium to produce a culture is ______.

Correct AnswerverifiedShow Answer

The bending of light rays as they pass from one medium to another is called refraction.

Correct AnswerverifiedShow Answer

A microbiologist makes a ﬁxed smear of bacterial cells and stains it with methylene blue. All cells appear blue under the oil immersion lens. This is an example of ______.

Correct AnswerverifiedShow Answer

The specimen preparation technique that is best for viewing cell motility is the ________.

Correct AnswerverifiedShow Answer

Basic dyes are _______.

Correct AnswerverifiedShow Answer

A pure culture contains _______.

Correct AnswerverifiedShow Answer

Which type of microscope bombards a whole, metal-coated specimen with electrons moving back and forth over it?

Correct AnswerverifiedShow Answer

What is the term for a culture made from one isolated colony?

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
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Correct Answer
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Correct Answer
verified

Correct Answer
verified

Magni?cation is achieved in a compound microscope through the initial magni?cation of the specimen by the _ lens. This image is then projected to the _ lens that will further magnify the specimen to form a virtual image received by the eye.

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
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Correct Answer
verified

Correct Answer
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