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Which of the following is an advantage to using phenolics as antimicrobial agents?


A) They are effective sterilants against all microbial forms.
B) They are not susceptible to resistance by organisms.
C) They have low toxicity.
D) The presence of organic matter does not limit its antimicrobial qualities.

E) A) and D)
F) C) and D)

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You have inherited some old glass baby milk bottles from your grandmother, and you would like to use them instead of plastic bottles. The bottles are placed into a large metal container and placed in the oven at 325oF for about 2 hours. What factor would you change if you wanted the sterilization to occur much quicker?


A) Use a pressure cooker to sterilize the bottles with steam
B) Place the bottles outside in the sunlight and then place in the oven
C) Pour an antimicrobial chemical into the bottles before placing into the oven
D) Increase the temperature of the oven by 5 degrees

E) C) and D)
F) B) and D)

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Which control method would not be a suitable choice for killing Mycobacterium in a capped culture tube?


A) Ultraviolet (germicidal) light
B) Gamma rays
C) 121°C at 15 psi for 15 minutes
D) 160°C for 2 hours
E) All of the choices are correct.

F) A) and C)
G) A) and E)

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A microorganism that is not motile and has stopped metabolizing is considered dead.

A) True
B) False

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_____ heat is more rapidly effective and e?cient compared to _____ heat.


A) High; dry
B) High; moist
C) Dry; moist
D) Moist; dry
E) Moist; high

F) A) and B)
G) A) and E)

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The easiest microbial forms to kill or inhibit are ______.


A) naked viruses
B) vegetative bacteria and fungi
C) endospores
D) protozoan cysts
E) mycobacteria and staphylococci

F) A) and E)
G) C) and E)

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The method of removing vegetative life forms from living surfaces is termed ______.


A) antisepsis
B) disinfection
C) sterilization
D) decontamination

E) B) and C)
F) C) and D)

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Most microbial contaminants of food are killed or inactivated at freezing temperatures.

A) True
B) False

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The use of chemical agents directly on exposed body surfaces to destroy or inhibit vegetative pathogens is _______.


A) disinfection
B) sterilization
C) antisepsis
D) sanitization

E) A) and D)
F) B) and C)

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You have some old plastic Petri dishes that you would like to use for pouring bacteriological agar plates. The only method of physical sterilization is ultraviolet radiation sterilization with your UV light (you cannot use the autoclave because it will dis?gure and melt the plastic) . After sterilizing the Plates, pouring the sterilized agar medium, and then leaving the plates out at room temperature for a day or two to let them solidify and dry, you ?nd contaminating bacterial colonies growing on the agar. Predict what has happened in this situation.


A) The lids of the Petri dishes were inadvertently left on while being sterilized.
B) The room temperature enhanced the growth of normal biota of the agar.
C) The agar had bacteria in it, even after sterilization in the autoclave.
D) Air got into the poured agar plates, contaminating them.

E) A) and C)
F) A) and B)

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The compound that is an organic base containing chlorine and two phenolic rings, and is used increasingly for wound degerming, neonatal washes, hand scrubbing, and prepping surgical skin sites is _______.


A) formalin
B) carbolic acid
C) chlorhexidine
D) triclosan
E) a quaternary ammonium compound

F) B) and D)
G) None of the above

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Using a HEPA filter in a vacuum or furnace is an example of which microbial control method?


A) Chemical agent
B) Gases
C) Surfactant
D) Dry control

E) All of the above
F) A) and D)

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Heavy metals work by _______.


A) mutating DNA
B) binding to DNA
C) dissolving the cell wall
D) inactivating proteins
E) rupturing the cell membrane

F) A) and B)
G) C) and D)

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Which of the following factors will influence the action of microbial agents?


A) The number of microorganisms
B) The type of microorganisms present
C) Temperature and pH
D) Mode and dosage of the agent
E) All of the choices will influence the action.

F) C) and D)
G) B) and C)

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Placing organisms at 4oC is ______.


A) bactericidal
B) bacteriostatic
C) decontamination
D) sterilization
E) germicidal

F) C) and D)
G) All of the above

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_____ radiation excites atoms to a higher energy state within molecules such as DNA, which leads to the formation of pyrimidine dimers.


A) Gamma
B) Infrared
C) Ionizing
D) Particle
E) Ultraviolet

F) None of the above
G) A) and B)

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Hydrogen peroxide is _______.


A) sporicidal
B) fungicidal
C) bactericidal
D) virucidal
E) All of the choices are correct.

F) D) and E)
G) C) and D)

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_____ solution was introduced in the late 19th century for preventing gonococcal infections in a newborn's eyes after exposure to the mother's infected birth canal.


A) Merthiolate
B) Zinc oxide
C) Silver nitrate
D) Triclosan
E) Betadine

F) B) and C)
G) C) and D)

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Which of the following is not a primary target of milk pasteurization?


A) Salmonella
B) Campylobacter jejuni
C) Lactobacillus
D) Listeria monocytogenes
E) Brucella

F) C) and D)
G) B) and C)

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What instrument is most effective for pressure-temperature sterilization?


A) Oven
B) Autoclave
C) Bunsen burner
D) Water-bath
E) Incubator

F) A) and E)
G) A) and C)

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