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Commercial sterilization differs from complete sterilization in that commercial sterilization


A) kills all microorganisms.
B) may result in the survival of thermophiles.
C) may result in the survival of fungal spores.
D) kills only bacteria.
E) employs a higher temperature.

F) A) and C)
G) A) and E)

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You are growing Bacillus subtilis, an aerobic bacterium that can also carry out fermentation when required, in a bioreactor and notice that the growth rate has slowed and the pH has decreased. You suspect the bacteria are


A) fermenting.
B) using proteins.
C) photosynthesizing.
D) using the Krebs cycle.
E) dead.

F) A) and D)
G) A) and C)

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Mesophilic bacteria grow best at temperatures of 60°C.

A) True
B) False

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Biomass is a renewable energy source derived from the organic matter produced by living organisms. All of the following are sources of biomass EXCEPT


A) algae.
B) oxidation of metals.
C) crops.
D) landfill sites.
E) bacteria.

F) A) and D)
G) C) and D)

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The longer interior-ripened cheeses are permitted to ripen, the milder the taste of the cheese.

A) True
B) False

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All of the following are advantages of a bioreactor instead of a flask culture EXCEPT


A) instrumentation for monitoring environmental conditions.
B) uniform aeration and mixing.
C) larger culture volumes can be grown.
D) aseptic sampling.
E) None of the answers is correct; all of these are advantages of using a bioreactor instead of a flask culture.

F) A) and D)
G) A) and E)

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Figure 28.1 Figure 28.1    -In Figure 28.1, assume the cells are producing penicillin which is collected during the idiophase. Remember, the top lines/markings indicate the different phases observed in the curve below them. When would you be able to collect the penicillin? A)  a B)  b C)  c D)  d -In Figure 28.1, assume the cells are producing penicillin which is collected during the idiophase. Remember, the top lines/markings indicate the different phases observed in the curve below them. When would you be able to collect the penicillin?


A) a
B) b
C) c
D) d

E) None of the above
F) A) and B)

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Acidithiobacillus ferrooxidans is used for the recovery of from ore.


A) gold
B) sulfur
C) sulfuric acid
D) iron
E) copper

F) A) and C)
G) C) and E)

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All of the following are industrial products produced using microbial fermentations EXCEPT


A) riboflavin.
B) citric acid.
C) glutamic acid.
D) aspartic acid.
E) saccharin.

F) B) and C)
G) B) and E)

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All of the following are industrial products produced by microbes EXCEPT


A) vitamin B12 and riboflavin.
B) industrial enzymes.
C) amino acids in food supplements.
D) uranium.
E) antibiotics.

F) A) and B)
G) C) and D)

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Which of the following reactions makes wine less acidic, and is important for good flavor when grapes with higher acidity are used?


A) carbon dioxide → sucrose
B) ethanol → acetic acid
C) sugar → Co2 + H2O
D) sugar → ethanol
E) malic acid → lactic acid

F) C) and D)
G) None of the above

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All of the following are true of irradiation methods used for food preservation and sterilization EXCEPT


A) they prolong shelf-life of fruits and vegetables.
B) they are used for the sterilization of many spices.
C) they greatly reduce the number of pathogens present in meats and poultry.
D) residual radioactivity is left behind on many foods.
E) ionizing radiation such as X rays or gamma rays is used.

F) C) and E)
G) A) and B)

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Aseptic packaging utilizes which of the following to sterilize laminated paper or plastic prior to filling with food?


A) hot hydrogen peroxide solution
B) hot HCl solution
C) autoclave
D) X rays
E) gamma radiation

F) B) and D)
G) A) and D)

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The following steps are required for making cheese. What is the second step, which helps to provide the characteristic flavors and aromas of the cheese?


A) enzymatic coagulation of milk
B) separation of curds and whey
C) inoculation with lactic acid bacteria
D) inoculation with Penicillium
E) fermentation of curd

F) A) and B)
G) D) and E)

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Microbial products can be improved by all of the following EXCEPT


A) isolating new strains.
B) mutating existing strains.
C) sterilization.
D) genetically modifying strains.
E) modifying culture conditions.

F) C) and E)
G) D) and E)

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Canning preserves food


A) by exposure to radiation.
B) by exposure to high pH.
C) by exposure to chemicals.
D) through use of aseptic packaging.
E) by exposure to high heat.

F) All of the above
G) B) and D)

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Figure 28.1 Figure 28.1    -In Figure 28.1, assume that a secondary metabolite is your desired product. Remember, the top lines/markings indicate the different phases observed in the curve below them. When would you be able to obtain it? A)  a B)  b C)  c D)  d -In Figure 28.1, assume that a secondary metabolite is your desired product. Remember, the top lines/markings indicate the different phases observed in the curve below them. When would you be able to obtain it?


A) a
B) b
C) c
D) d

E) B) and C)
F) A) and D)

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Which of the following microorganisms is usually used in the fermentation of alcoholic beverages?


A) Lactobacillus
B) Saccharomyces
C) Propionobacterium
D) Rhizopus
E) Penicillium

F) C) and E)
G) B) and E)

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Canning works to preserve foods through


A) increasing pH.
B) creation of an anaerobic environment.
C) exposure to high heat.
D) mutation of bacterial DNA.
E) creation of an anaerobic environment and exposure to high heat.

F) A) and E)
G) B) and E)

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The blue-green clumps observed in Roquefort cheese are a result of mold growth.

A) True
B) False

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