Question 1

A pure culture contains only

Accepted Answer

A)   one species of microorganism.
B)   bacteria.
C)   a variety of microbes from one source.
D)   All of these choices are correct.E) A) and B)
F) A) and C)

Question 2

Which of the following characteristics refers to the microscope's ability to show two separate entities as separate and distinct?

Accepted Answer

A)   resolving power
B)   magnification
C)   refraction
D)   All of these choices are correct.E) A) and B)
F) B) and C)

Question 3

Explain the difference and significance between a contaminated culture and a mixed culture.

Accepted Answer

A contaminated culture refers to a culture that has been unintentionally contaminated with microorganisms from the environment, such as bacteria, fungi, or other contaminants. This can occur during the process of inoculating the culture or due to improper handling or sterilization techniques. Contaminated cultures can lead to inaccurate results and can compromise the integrity of the experiment or study being conducted.

On the other hand, a mixed culture refers to a culture that contains more than one type of microorganism. This can occur intentionally, such as when studying the interactions between different species, or unintentionally, such as when a culture becomes contaminated. Mixed cultures can be significant in research and clinical settings as they can provide insight into how different microorganisms interact and compete with each other.

The significance of distinguishing between contaminated and mixed cultures lies in the accuracy and reliability of the results obtained from the culture. Contaminated cultures can lead to false conclusions and unreliable data, while mixed cultures can provide valuable information about microbial interactions. Proper handling and sterilization techniques are essential to prevent contamination and ensure the integrity of the culture being studied.

Question 4

Which microscope achieves the greatest resolution and highest magnification?

Accepted Answer

A)   bright-field
B)   dark-field
C)   phase-contrast
D)   fluorescence
E)   electronF) A) and B)
G) A) and C)

Question 5

A reducing medium contains

Accepted Answer

A)   sugars that can be fermented.
B)   extra oxygen.
C)   hemoglobin, vitamins, or other growth factors.
D)   substances that remove oxygen.
E)   inhibiting agents.F) A) and B)
G) A) and C) D

Question 6

Brain-heart infusion, trypticase soy agar TSA) , and nutrient agar are all examples of which type of media?

Accepted Answer

A)   synthetic
B)   reducing
C)   enriched
D)   complex
E)   selectiveF) A) and B)
G) C) and E) D

Question 7

The primary purpose of staining cells on a microscope slide is to

Accepted Answer

A)   kill them.
B)   secure them to the slide.
C)   enlarge the cells.
D)   add contrast in order to see them better.
E)   see motility.F) A) and B)
G) A) and C)

Question 8

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Accepted Answer

A)   differential
B)   selective
C)   enumeration
D)   enriched
E)   reducingF) A) and B)
G) A) and C)

Question 9

The correct microbiological term for the tiny sample of specimen that is put into a nutrient medium in order to produce a culture is the _____.

Accepted Answer

The answer of The correct microbiological term for the tiny...

Question 10

The _____ of the microscope holds and allows selection of the objective lenses.

Accepted Answer

The answer of The _____ of the microscope holds and...

Question 11

Which of the following places the steps for microbial culture and identification in the correct order?

Accepted Answer

A)   inoculation, incubation, isolation, inspection, identification
B)   isolation, inspection, inoculation, incubation, identification
C)   incubation, inspection, isolation, identification, inoculation
D)   inspection, identification, isolation, incubation, inoculation
E)   inspection, isolation, incubation, inoculation, identificationF) B) and C)
G) B) and D)

Question 12

A medium that is gel-like has less agar in it compared to a solid medium.

Accepted Answer

A) True 
 B)False

Question 13

All of the following are diameters of cells that would be resolved in a microscope with a limit of resolution of 0.2 µm except

Accepted Answer

A)   0.2 µm.
B)   0.2 mm.
C)   0.1 µm.
D)   0.3 µm.
E)   2.0 µm.F) A) and B)
G) None of the above

Question 14

Mannitol salt agar is selective for which bacterial genus?

Accepted Answer

A)   Salmonella
B)   Streptococcus
C)   Neisseria
D)   Staphylococcus
E)   EscherichiaF) A) and D)
G) B) and E)

Question 15

A contaminated food sample contains several different species of bacteria. A food microbiologist is interested in studying just one of these species. Describe the sequence of procedures that the microbiologist must perform in order to obtain a pure culture of the bacterial species of interest from this food sample. Detail all the necessary media and equipment.

Accepted Answer

The microbiologist must first obtain a s...

Question 16

Which of the following magnifies the specimen to produce the real image of the specimen?

Accepted Answer

A)   condenser
B)   objective lens
C)   ocular lens
D)   body
E)   nosepieceF) None of the above
G) A) and D)

Question 17

Bacteria that require special growth factors and complex nutrients are termed _____.

Accepted Answer

The answer of Bacteria that require special growth factors and...

Question 18

A common medium used for growing fastidious bacteria is

Accepted Answer

A)   chocolate agar.
B)   trypticase soy agar.
C)   mannitol salt agar.
D)   MacConkey medium.
E)   a reducing medium.F) D) and E)
G) A) and B)

Question 19

A media is designed that allows only staphylococci to grow. In addition, S. aureus colonies have a yellow halo around them and other staphylococci appear white. This type of media is

Accepted Answer

A)   selective only.
B)   differential only.
C)   both selective and differential.
D)   a reducing media.
E)   enriched.F) A) and B)
G) A) and E)

Question 20

Compare and contrast the reagents and functions of negative staining versus positive staining.

Accepted Answer

Negative staining and positive staining ...

Exam 3: Tools of the Laboratory: Methods of Studying Microorganisms

A pure culture contains only

Correct Answer
verified

Which of the following characteristics refers to the microscope's ability to show two separate entities as separate and distinct?

Correct Answer
verified

Explain the difference and significance between a contaminated culture and a mixed culture.

Which microscope achieves the greatest resolution and highest magnification?

Correct Answer
verified

A reducing medium contains

Correct Answer
verified
D

Brain-heart infusion, trypticase soy agar TSA) , and nutrient agar are all examples of which type of media?

Correct Answer
verified
D

The primary purpose of staining cells on a microscope slide is to

Correct Answer
verified

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Correct Answer
verified

The correct microbiological term for the tiny sample of specimen that is put into a nutrient medium in order to produce a culture is the _____.

Correct Answer
verified

The _____ of the microscope holds and allows selection of the objective lenses.

Correct Answer
verified

Which of the following places the steps for microbial culture and identification in the correct order?

Correct Answer
verified

A medium that is gel-like has less agar in it compared to a solid medium.

Correct Answer
verified

All of the following are diameters of cells that would be resolved in a microscope with a limit of resolution of 0.2 µm except

Correct Answer
verified

Mannitol salt agar is selective for which bacterial genus?

Correct Answer
verified

Correct Answer
Answered by ExamLex AI
The microbiologist must first obtain a s...
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Which of the following magnifies the specimen to produce the real image of the specimen?

Correct Answer
verified

Bacteria that require special growth factors and complex nutrients are termed _____.

Correct Answer
verified

A common medium used for growing fastidious bacteria is

Correct Answer
verified

A media is designed that allows only staphylococci to grow. In addition, S. aureus colonies have a yellow halo around them and other staphylococci appear white. This type of media is

Correct Answer
verified

Compare and contrast the reagents and functions of negative staining versus positive staining.

Correct Answer
Answered by ExamLex AI
Negative staining and positive staining ...
View Answer

Exam 3: Tools of the Laboratory: Methods of Studying Microorganisms

A pure culture contains only

Correct AnswerverifiedShow Answer

Which of the following characteristics refers to the microscope's ability to show two separate entities as separate and distinct?

Correct AnswerverifiedShow Answer

Explain the difference and significance between a contaminated culture and a mixed culture.

Which microscope achieves the greatest resolution and highest magnification?

Correct AnswerverifiedShow Answer

A reducing medium contains

Correct AnswerverifiedD

Brain-heart infusion, trypticase soy agar TSA) , and nutrient agar are all examples of which type of media?

Correct AnswerverifiedD

The primary purpose of staining cells on a microscope slide is to

Correct AnswerverifiedShow Answer

Which type of medium is able to distinguish different species or types of microorganisms based on an observable change in the colonies or in the medium?

Correct AnswerverifiedShow Answer

The correct microbiological term for the tiny sample of specimen that is put into a nutrient medium in order to produce a culture is the _____.

Correct AnswerverifiedShow Answer

The _____ of the microscope holds and allows selection of the objective lenses.

Correct AnswerverifiedShow Answer

Which of the following places the steps for microbial culture and identification in the correct order?

Correct AnswerverifiedShow Answer

A medium that is gel-like has less agar in it compared to a solid medium.

Correct AnswerverifiedShow Answer

All of the following are diameters of cells that would be resolved in a microscope with a limit of resolution of 0.2 µm except

Correct AnswerverifiedShow Answer

Mannitol salt agar is selective for which bacterial genus?

Correct AnswerverifiedShow Answer

Correct AnswerAnswered by ExamLex AIThe microbiologist must first obtain a s...View AnswerShow Answer

Which of the following magnifies the specimen to produce the real image of the specimen?

Correct AnswerverifiedShow Answer

Bacteria that require special growth factors and complex nutrients are termed _____.

Correct AnswerverifiedShow Answer

A common medium used for growing fastidious bacteria is

Correct AnswerverifiedShow Answer

A media is designed that allows only staphylococci to grow. In addition, S. aureus colonies have a yellow halo around them and other staphylococci appear white. This type of media is

Correct AnswerverifiedShow Answer

Compare and contrast the reagents and functions of negative staining versus positive staining.

Correct AnswerAnswered by ExamLex AINegative staining and positive staining ...View AnswerShow Answer

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D

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Answered by ExamLex AI
The microbiologist must first obtain a s...
View Answer

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Correct Answer
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Correct Answer
Answered by ExamLex AI
Negative staining and positive staining ...
View Answer