Question 1

A branched ("lariat") structure is formed during:

Accepted Answer

A)  attachment of a 5' cap to mRNA.
B)  attachment of poly(
A)  tails to mRNA.
C)  processing of preribosomal RNA.
D)  splicing of all classes of introns.
E)  splicing of group II introns.F) A) and B)
G) A) and C)

Question 2

List one basic property that distinguishes RNA polymerases from DNA polymerases,and list one basic property they share.

Accepted Answer

Among the distinguishing characteristics...

Question 3

After binding by E.coli RNA polymerase,the correct order of events for transcription initiation is:

Accepted Answer

A)  closed complex formation,open complex formation,promoter clearance,start of RNA synthesis.
B)  closed complex formation,open complex formation,start of RNA synthesis,promoter clearance.
C)  open complex formation,closed complex formation,start of RNA synthesis,promoter clearance.
D)  start of RNA synthesis,closed complex formation,open complex formation,promoter clearance.
E)  start of RNA synthesis,open complex formation,closed complex formation,promoter clearance.F) A) and B)
G) C) and D)

Question 4

Define ribozymes and briefly describe the structure and function of two ribozymes.

Accepted Answer

Ribozymes are enzymes that consist in pa...

Question 5

Which of the following is not usually essential for the catalytic activity of ribozymes?

Accepted Answer

A)  Correct base pairing
B)  Correct base sequence
C)  Correct interaction with protein
D)  Correct secondary structure
E)  Correct three-dimensional structureF) A) and B)
G) All of the above

Question 6

The excision (splicing) of many group I introns requires,in addition to the primary transcript RNA:

Accepted Answer

A)  a cytosine nucleoside or nucleotide and a protein enzyme.
B)  a guanine nucleoside or nucleotide (only) .
C)  a protein enzyme only.
D)  a small nuclear RNA and a protein enzyme.
E)  ATP,NAD,and a protein enzyme.F) All of the above
G) A) and C)

Question 7

Which of the following is not part of miRNA formation?

Accepted Answer

A)  Pri-miRNA
B)  Pre-miRNA
C)  Dicer
D)  RISC
E)  PseudouridineF) A) and B)
G) A) and C)

Question 8

Describe briefly the process of initiation by eukaryotic RNA polymerase.

Accepted Answer

One transcription factor (TBP)binds spec...

Question 9

Splicing of introns in nuclear mRNA primary transcripts requires:

Accepted Answer

A)  a guanine nucleoside or nucleotide.
B)  endoribonucleases.
C)  polynucleotide phosphorylase.
D)  RNA polymerase II.
E)  small nuclear ribonucleoproteins (snRNPs) .F) A) and D)
G) None of the above

Question 10

Which of the following is not a post-transcriptional modification in mRNA or tRNA?

Accepted Answer

A)  4-thiouridine
B)  Pseudouridine
C)  Dihydrouridine
D)  Ribouridine
E)  RibothymidineF) A) and C)
G) C) and D)

Question 11

Describe the sequence of events in the initiation of transcription by E.coli RNA polymerase.

Accepted Answer

The core enzyme plus <img  loading="lazy" src="/assets/images/blured-textbook.svg" class="d-inline m-1" alt="blured image" width="139" height="30"> subunit,called hol...

Question 12

In 1989,Sidney Altman won a Nobel Prize for his work on RNase P.In no more than three sentences,describe the function of RNase P,as well as the unusual characteristic of this enzyme that made Altman's work so important.

Accepted Answer

RNase P catalyzes the removal ...

Question 13

RNA polymerase from E.coli (core enzyme alone) has all of the following properties except that it:

Accepted Answer

A)  can extend an RNA chain and initiate a new chain.
B)  is required for the synthesis of mRNA,rRNA,and tRNA in E.coli.
C)  produces an RNA polymer that begins with a 5'-triphosphate.
D)  recognizes specific start signals in DNA.
E)  requires all four ribonucleoside triphosphates and a DNA template.F) A) and B)
G) B) and D)

Question 14

Below,an RNA molecule is being transcribed from a strand of DNA.Indicate the 5' and 3' ends of the RNA molecule and of the strand of DNA that is complementary to the RNA molecule.In which direction is synthesis occurring?

Accepted Answer

The answer of Below,an RNA molecule is being transcribed from...

Question 15

Which of these polymerases does not require a template?

Accepted Answer

A)  RNA pol I
B)  RNA pol II
C)  Reverse transcriptase
D)  Polyadenylate polymerase
E)  RNA replicaseF) A) and D)
G) C) and D)

Question 16

In a $\rho$-independent terminator,there is a palindrome rich in G$ \equiv$C base pairs,followed by 8-10 uridine residues.Explain how each of the following changes might affect terminator function: 
(a)substitution of cytidines for the 8-10 uridines; 
(b)mutations in the palindrome that decrease its G$ \equiv$C content; 
(c)elimination of half of the palindromic sequence.

Accepted Answer

(a)This substitution would decrease term...

Question 17

"Footprinting," or DNase protection,is a technique used to identify:

Accepted Answer

A)  a region of DNA that has been damaged by mutation.
B)  
E)  coli cells that contain a desired,cloned piece of DNA.
C)  the position of a particular gene of a chromosome.
D)  the position of internally double-stranded regions in a single-stranded DNA molecule.
E)  the specific binding site of a repressor,polymerase,or other protein on the DNA.F) A) and E)
G) A) and D)

Question 18

Describe the mechanistic difference that distinguishes the splicing of group I introns from that of group II introns.

Accepted Answer

In the group I introns,the initial break...

Exam 26: Rna Metabolism

Below,an RNA molecule is being transcribed from a strand of DNA.Indicate the 5' and 3' ends of the RNA molecule and of the strand of DNA that is complementary to the RNA molecule.In which direction is synthesis occurring?

Correct Answer
verified

Describe the mechanistic difference that distinguishes the splicing of group I introns from that of group II introns.

Correct Answer
verified
In the group I introns,the initial break...
View Answer

Which of these polymerases does not require a template?

Correct Answer
verified

Describe the sequence of events in the initiation of transcription by E.coli RNA polymerase.

Correct Answer
verified
The core enzyme plus subunit,called hol...
View Answer

The excision (splicing) of many group I introns requires,in addition to the primary transcript RNA:

Correct Answer
verified

Which of the following is not a post-transcriptional modification in mRNA or tRNA?

Correct Answer
verified

"Footprinting," or DNase protection,is a technique used to identify:

Correct Answer
verified

A branched ("lariat") structure is formed during:

Correct Answer
verified

Correct Answer
verified
(a)This substitution would decrease term...
View Answer

Define ribozymes and briefly describe the structure and function of two ribozymes.

Correct Answer
verified
Ribozymes are enzymes that consist in pa...
View Answer

RNA polymerase from E.coli (core enzyme alone) has all of the following properties except that it:

Correct Answer
verified

Describe briefly the process of initiation by eukaryotic RNA polymerase.

Correct Answer
verified
One transcription factor (TBP)binds spec...
View Answer

After binding by E.coli RNA polymerase,the correct order of events for transcription initiation is:

Correct Answer
verified

Which of the following is not part of miRNA formation?

Correct Answer
verified

In 1989,Sidney Altman won a Nobel Prize for his work on RNase P.In no more than three sentences,describe the function of RNase P,as well as the unusual characteristic of this enzyme that made Altman's work so important.

Correct Answer
verified
RNase P catalyzes the removal ...
View Answer

Splicing of introns in nuclear mRNA primary transcripts requires:

Correct Answer
verified

List one basic property that distinguishes RNA polymerases from DNA polymerases,and list one basic property they share.

Correct Answer
verified
Among the distinguishing characteristics...
View Answer

Which of the following is not usually essential for the catalytic activity of ribozymes?

Correct Answer
verified

Exam 26: Rna Metabolism

Below,an RNA molecule is being transcribed from a strand of DNA.Indicate the 5' and 3' ends of the RNA molecule and of the strand of DNA that is complementary to the RNA molecule.In which direction is synthesis occurring?

Correct AnswerverifiedShow Answer

Describe the mechanistic difference that distinguishes the splicing of group I introns from that of group II introns.

Correct AnswerverifiedIn the group I introns,the initial break...View AnswerShow Answer

Which of these polymerases does not require a template?

Correct AnswerverifiedShow Answer

Describe the sequence of events in the initiation of transcription by E.coli RNA polymerase.

Correct AnswerverifiedThe core enzyme plus subunit,called hol...View AnswerShow Answer

The excision (splicing) of many group I introns requires,in addition to the primary transcript RNA:

Correct AnswerverifiedShow Answer

Which of the following is not a post-transcriptional modification in mRNA or tRNA?

Correct AnswerverifiedShow Answer

"Footprinting," or DNase protection,is a technique used to identify:

Correct AnswerverifiedShow Answer

A branched ("lariat") structure is formed during:

Correct AnswerverifiedShow Answer

Correct Answerverified(a)This substitution would decrease term...View AnswerShow Answer

Define ribozymes and briefly describe the structure and function of two ribozymes.

Correct AnswerverifiedRibozymes are enzymes that consist in pa...View AnswerShow Answer

RNA polymerase from E.coli (core enzyme alone) has all of the following properties except that it:

Correct AnswerverifiedShow Answer

Describe briefly the process of initiation by eukaryotic RNA polymerase.

Correct AnswerverifiedOne transcription factor (TBP)binds spec...View AnswerShow Answer

After binding by E.coli RNA polymerase,the correct order of events for transcription initiation is:

Correct AnswerverifiedShow Answer

Which of the following is not part of miRNA formation?

Correct AnswerverifiedShow Answer

In 1989,Sidney Altman won a Nobel Prize for his work on RNase P.In no more than three sentences,describe the function of RNase P,as well as the unusual characteristic of this enzyme that made Altman's work so important.

Correct AnswerverifiedRNase P catalyzes the removal ...View AnswerShow Answer

Splicing of introns in nuclear mRNA primary transcripts requires:

Correct AnswerverifiedShow Answer

List one basic property that distinguishes RNA polymerases from DNA polymerases,and list one basic property they share.

Correct AnswerverifiedAmong the distinguishing characteristics...View AnswerShow Answer

Which of the following is not usually essential for the catalytic activity of ribozymes?

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified
In the group I introns,the initial break...
View Answer

Correct Answer
verified

Correct Answer
verified
The core enzyme plus subunit,called hol...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
(a)This substitution would decrease term...
View Answer

Correct Answer
verified
Ribozymes are enzymes that consist in pa...
View Answer

Correct Answer
verified

Correct Answer
verified
One transcription factor (TBP)binds spec...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
RNase P catalyzes the removal ...
View Answer

Correct Answer
verified

Correct Answer
verified
Among the distinguishing characteristics...
View Answer

Correct Answer
verified